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avidin biotin complex kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin biotin complex kit
    Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12779 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hrp+complex+vectastain+elite+abc+hrp+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13122667-89-14-17
    Average 99 stars, based on 12779 article reviews
    avidin biotin complex kit - by Bioz Stars, 2026-10
    99/100 stars

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    Incubation:

    Article Title: Small heterodimer partner protects against osteoarthritis by inhibiting IKKβ/NF-κB-mediated matrix-degrading enzymes in chondrocytes.
    Article Snippet: .. The sections were incubated with biotinylated horse anti-mouse IgG or goat anti-rabbit IgG secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 1 h, followed by incubation with avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain Elite ABC-HRP kit, PK-6101, Vector Laboratories) at room temperature for 30 min. .. The signal was developed with the 3,3′-diaminobenzidine (DAB) chromogen (DAB Peroxidase HRP Substrate Kit; SK-4100; Vector Laboratories), and cell nuclei were labelled with haematoxylin.

    Article Title: The secreted protein Amuc_1409 from Akkermansia muciniphila improves gut health through intestinal stem cell regulation.
    Article Snippet: The sections were incubated with a monoclonal rabbit anti-Ki67 antibody (Abcam, Cambridge, UK) or anti-OLFM4 antibody (Cell Signaling Technology, Danvers, MA, USA) at 4 °C overnight. .. The sections were incubated with biotinylated goat anti-rabbit IgG secondary antibody (Vector Laboratories, Burlingame, CA, USA) at room temperature for 1 h, followed by incubation with avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain Elite ABC-HRP kit, PK-6101, Vector Laboratories) at room temperature for 30min. .. The signals were developed with the 3,3′-diaminobenzidine (DAB) chromogen (DAB Peroxidase HRP Substrate Kit; SK-4100; Vector Laboratories), and cell nuclei were labeled with haematoxylin.

    Avidin-Biotin Assay:

    Article Title: Small heterodimer partner protects against osteoarthritis by inhibiting IKKβ/NF-κB-mediated matrix-degrading enzymes in chondrocytes.
    Article Snippet: .. The sections were incubated with biotinylated horse anti-mouse IgG or goat anti-rabbit IgG secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 1 h, followed by incubation with avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain Elite ABC-HRP kit, PK-6101, Vector Laboratories) at room temperature for 30 min. .. The signal was developed with the 3,3′-diaminobenzidine (DAB) chromogen (DAB Peroxidase HRP Substrate Kit; SK-4100; Vector Laboratories), and cell nuclei were labelled with haematoxylin.

    Article Title: The secreted protein Amuc_1409 from Akkermansia muciniphila improves gut health through intestinal stem cell regulation.
    Article Snippet: The sections were incubated with a monoclonal rabbit anti-Ki67 antibody (Abcam, Cambridge, UK) or anti-OLFM4 antibody (Cell Signaling Technology, Danvers, MA, USA) at 4 °C overnight. .. The sections were incubated with biotinylated goat anti-rabbit IgG secondary antibody (Vector Laboratories, Burlingame, CA, USA) at room temperature for 1 h, followed by incubation with avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain Elite ABC-HRP kit, PK-6101, Vector Laboratories) at room temperature for 30min. .. The signals were developed with the 3,3′-diaminobenzidine (DAB) chromogen (DAB Peroxidase HRP Substrate Kit; SK-4100; Vector Laboratories), and cell nuclei were labeled with haematoxylin.

    Microscopy:

    Article Title: GABA co-released from striatal dopamine axons dampens phasic dopamine release through autoregulatory GABA A receptors
    Article Snippet: The secondary antibody for α 3 was biotinylated donkey anti-rabbit (1:200) (catalog #711-065-152, Jackson ImmunoResearch Laboratories) and for TH it was donkey anti-mouse IgG conjugated to ultrasmall (0.8 nm) colloidal gold (1:40) (catalog #25810, temporary RRID: AB_2631210; Electron Microscopy Sciences). .. Sections were treated with 3,3′-Diaminobenzidine (DAB, Sigma Aldrich, St. Louis, MO, USA) plus avidinbiotin-horseradish-peroxidase (HRP) complex (Vectastain Elite ABC-HRP Kit, catalog #PK6100, Vector Laboratories, Burlingame, CA, USA) to visualize α3 and silver intensified using a Silver Enhancer Kit for Microscopy (catalog #50-22-01; Kirkegaard & Perry Laboratories (KPL), Inc., Gaithersburg, MD, USA) for 17 min to enlarge the colloidal gold particles (silver-intensified gold, SIG) for visualizing TH, and processed for EM as previously., Ultra-thin sections were cut from sections embedded in resin and collected onto EM grids. .. Digital images to quantify co-localization were captured by a 1.2-megapixel Hamamatsu CCD camera (AMT, Boston, MA, USA) attached to a JEOL 1200XL electron microscope and running AMT’s software.

    other:

    Article Title: GABA co-released from striatal dopamine axons dampens phasic dopamine release through autoregulatory GABA A receptors.
    Article Snippet: Ai32; DAT-cre mice Mice expressing a blue light sensitive ChR2 (H134R variant) selectively in DA neurons were generated as previously.60 Breeder knock-in mouse lines were originally purchased from The Jackson Laboratory (Bar Harbor, ME, USA).



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